角膜上皮片及其制作方法

Corneal epithelial sheet and process for producing the same

Abstract

A corneal epithelial sheet having a cell layer similar to the anterior epithelium of cornea. A corneal epithelial sheet is obtained through the step (a) of separately preparing a first cell being a self cell and a second cell whose origin is different from that of the first cell, the step (b) of planting the first cell and the second cell on a collagen layer and culturing them, and the step (c) of after proliferation of the first cell and second cell resulting in formation of a cell layer, bringing the outermost stratum of the cell layer into contact with air.
提供具有类似角膜上皮的细胞层的角膜上皮片。进行如下步骤得到角膜上皮片:a)分别调制作为自体细胞的第1细胞以及与该第1细胞来源不同的第2细胞的步骤;b)把上述第1细胞以及上述第2细胞播种于胶原层上进行培养的步骤;c)上述第1细胞以及上述第2细胞增殖形成细胞层后,把该细胞层的最表层与空气进行接触的步骤。

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    CN-105308175-AFebruary 03, 2016日本化学研究株式会社, 京都府公立大学法人Method for producing human corneal epithelium sheet